This guide is for general information only and is not legal advice. Laws change — verify current rules with your state DOT or a licensed attorney before relying on this for any legal matter. Read full disclaimer.
The rule
Bicycles are prohibited on Interstate highways in Florida. Bicycles are prohibited on all Interstate highways in Florida (I-4, I-10, I-75, I-95, I-275, I-295, I-595) under FDOT Rule 14-65.0031 and the on-ramp signage. Conventional US routes and state highways — including the long-distance touring routes across the Panhandle and through the Keys — are open by default.
What this means in practice
Stick to surface streets, US routes, state highways, and dedicated paths. If your route in Florida appears to use an Interstate, plan a detour before you start.