This guide is for general information only and is not legal advice. Laws change — verify current rules with your state DOT or a licensed attorney before relying on this for any legal matter. Read full disclaimer.
The rule
Bicycles are prohibited on Interstate highways in Wisconsin. Wis. Stat. § 346.075 and Wis. Admin. Code Trans 276 prohibit bicycles, pedestrians and other non-motorized traffic on Wisconsin Interstate highways and other expressways designated as freeways. I-39, I-41, I-43, I-90, I-94 and I-535 are signed closed at on-ramps.
What this means in practice
Stick to surface streets, US routes, state highways, and dedicated paths. If your route in Wisconsin appears to use an Interstate, plan a detour before you start.