This guide is for general information only and is not legal advice. Laws change — verify current rules with your state DOT or a licensed attorney before relying on this for any legal matter. Read full disclaimer.
The rule
Bicycles are prohibited on Interstate highways in Missouri. Bicycles are prohibited on Missouri Interstate and other limited-access highways within urban areas under Missouri Department of Transportation (MoDOT) controlled-access regulations. I-70, I-44, I-55, I-29, and I-35 are closed to bicycles end-to-end across the state. Conventional US and state routes (US-50, US-63, MO-94 Katy Trail parallel) remain open.
What this means in practice
Stick to surface streets, US routes, state highways, and dedicated paths. If your route in Missouri appears to use an Interstate, plan a detour before you start.